#5485 CHRONIC KIDNEY DISEASE NOR STROMAL CELL-DERIVED FACTOR 1Α PEPTIDE (SDF-1A) BIO-FUNCTIONALIZATION ALTER RODENT VASCULAR IN SITU TISSUE FORMATION

Abstract Background and Aims The systemic pro-inflammatory environment of chronic kidney disease (CKD) could influence neo-tissue formation in situ tissue engineered vascular access grafts. Here, we studied in-graft inflammation a rat CKD model. Moreover, explored graft bio-functionalization with stromal cell-derived factor 1α peptides (SDF-1α), progenitor cell chemotactic which may improve engraftment. Method Pristine or SDF-1α bio-functionalized grafts (1,2 mm ID, 2 cm length, 250–300 μm wall thickness) were created from biodegradable, polycarbonate-bisurea (PC-BU) electrospun meshes. Abdominal aorta interposition implanted female Sprague Dawley rats (n = 53) that underwent sham surgery induction by 5/6th nephrectomy. Explanations analyses performed after two 25) twelve 9) weeks. Results At weeks, survival patency 100%. Explant cellularity collagen content not significantly different between influenced SDF-1α. Endothelial coverage (RECA+) smooth muscle presence (αSMA+) visually similar irrespective bio-functionalization. Elastin+ (p 0,11), pan-(CD68+, p 0,08) anti-inflammatory macrophage (CD163+, 0,52) surface area, as well (anti-)inflammatory gene expression groups. twelve-week CKD-group was taken out experiment prematurely due to rapid progression. In addition, preliminary death rupture occurred four animals pristine n SDF-1α) the animals. While patent, all remaining explants at weeks showed severe dilatation calcifications (Von Kossa+). No differences inflammatory markers found over time. Conclusion did alter Additionally, engraftment formation. Mechanical stability appears be primary driver Future emphasis on vitro vivo translation should fine balance one hand fiber resorption other hand.